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Artigo

A novel Moringa oleifera leaf extract can mitigate the stress effects of salinity and cadmium in bean (Phaseolus vulgaris L.) plants

Resumo

Plantas de Phaseolus vulgaris foram cultivadas na presença de NaCl e/ou CdCl2 a partir da segunda semana, pulverizadas duas vezes com extrato de folha de moringa (MLE) aos 21 e 28 dias após a semeadura (DAS), e foram amostradas aos 35 DAS para crescimento e química análises e cedeu ao final do experimento. As características de crescimento, nível de pigmentos fotossintéticos, produção de vagens verdes e proteína das vagens foram significativamente reduzidas com a exposição das plantas a NaCl e/ou CdCl2. No entanto, a aplicação foliar de acompanhamento com MLE desintoxica o estresse gerado por NaCl e/ou CdCl2 e aumenta significativamente os parâmetros acima mencionados. Os estresses individuais ou combinados aumentaram o vazamento de eletrólitos (EL), a peroxidação lipídica e o teor de Cd(2+) da planta, e diminuíram o índice de estabilidade da membrana (MSI) e o teor relativo de água (RWC). No entanto, a aplicação foliar de MLE na ausência do estresse melhorou o MSI e o RWC e minimizou o teor de Cd(2+) da planta, mas não afetou o EL e a peroxidação lipídica. O conteúdo de prolina e a atividade de enzimas antioxidantes mostraram um aumento significativo em resposta ao estresse por MLE, bem como por NaCl e/ou CdCl2.



Abstract

Phaseolus vulgaris plants were grown in the presence of NaCl and/or CdCl2 beginning from the second week, sprayed twice with moringa leaf extract (MLE) at 21 and 28 days after sowing (DAS), and were sampled at 35 DAS for growth and chemical analyses and yielded at the end of experiment. Growth traits, level of photosynthetic pigments, green pod yield and pod protein were significantly reduced with exposing the plants to NaCl and/or CdCl2. However, the follow up foliar application with MLE detoxified the stress generated by NaCl and/or CdCl2 and significantly enhanced the aforementioned parameters. Either individual or combined used stresses increased the electrolyte leakage (EL), lipid peroxidation and plant Cd(2+) content, and decreased the membrane stability index (MSI) and relative water content (RWC). However, the foliar application of MLE in the absence of the stress improved the MSI and RWC and minimized plant Cd(2+) content but could not affect EL and lipid peroxidation. Proline content and the activity of antioxidant enzymes showed a significant increase in response to MLE as well as to NaCl and/or CdCl2 stress.



Saad M. Howladar

2014 - Ecotoxicology and Environmental Safety

Palavras-chave:

Antioxidant system, Beans, Cadmium stress, Growth, Moringa leaf extract, Salinity.

Termos de indexação:

bioensaios

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