Dynamic changes in enzyme activities and phenolic content during in vitro rooting of tree peony (Paeonia suffruticosa Andr.) plantlets

Resumo

As mudanças dinâmicas de conteúdo fenólico e peroxidase (POD), polifenol
oxidase (PPO), ácido indol-3-acético oxidase (IAAO) e fenilalanina amônia liase
(PAL) foram avaliadas durante o processo de enraizamento in vitro de três cultivares de árvores
peônia (Paeonia suffruticosa Andr.). Essas mudanças na atividade relacionada à enzima e
conteúdo fenólico observado ao nível de toda a planta diferiu durante os primeiros 20 dias
do processo de enraizamento em cultivares ‘Feng Dan Bai’ de fácil enraizamento e ‘Wu
Cultivares Long Peng Sheng’ e ‘Tai Ping Hong’, e na maioria dos casos eram realmente
oposto. A facilidade com que ‘Feng Dan Bai’ conseguia enraizar estava intimamente relacionada com a
atividade de todas as quatro enzimas (POD, PPO, IAAO, PAL), bem como ao conteúdo fenólico.

Abstract

The dynamic changes of phenolic content and peroxidase (POD), polyphenol
oxidase (PPO), indole-3-acetic acid oxidase (IAAO) and phenylalanine ammonia lyase
(PAL) activities were assessed during the in vitro rooting process of three cultivars of tree
peony (Paeonia suffruticosa Andr.). These changes in enzyme-related activity and
phenolic content__observed at the level of the whole plant__differed during the first 20 days
of the rooting process in easy-to-root ‘Feng Dan Bai’ cultivar and difficult-to-root ‘Wu
Long Peng Sheng’ and ‘Tai Ping Hong’ cultivars, and in most cases they were actually
opposite. The ease with which ‘Feng Dan Bai’ was able to root was closely related to the
activity of all four enzymes (POD, PPO, IAAO, PAL) as well as to the phenolic content.