GC-MS analysis and antibacterial activity of Cuscuta reflexa against bacterial pathogens

Resumo

Objetivo: Conhecer a eficácia antibacteriana da Cuscuta reflexa (C. reflexa) e examinar a composição química do óleo essencial de C. reflexa por cromatografia gasosa e massa espectrometria (GC-MS). Métodos: A potência antibacteriana dos extratos vegetais de C. reflexa foi testada contra Bacillus subtilis IFO 3026, Sarcina lutea IFO 3232, Xanthomonas campestris IAM 1671, Escherichia coli IFO 3007, Klebsiella pneumoniae ATTC 10031, Proteus vulgaris MTCC 321 e Pseudomonas denitrificans KACC 32026 por ensaio de difusão em disco. A composição química dos essenciais óleo foram analisados ​​por GC-MS. Resultados: A análise GC-MS identificou dezesseis compostos do óleo essencial de C. reflexa, de qual piperitona (15,19%), cariofileno (12,55%), β-elemeno (10,35%), elemol (6,20%), α-selineno (4,49%), α-humuleno (3,98%) e tujona (5,18%) foram os compostos majoritários. ααBergamoteno e cânfora de zimbro foram identificados como os compostos menores. Diclorometano e extratos de éter de petróleo de C. reflexa mostraram um grande potencial de atividade antibacteriana contra todas as bactérias testadas. Valores mínimos de concentração inibitória de vários extratos variaram de 16 a 512 µg/mL. Conclusões: Esses extratos mostraram boa atividade antibacteriana e podem levar a novos projeto de drogas antibacterianas.

Abstract

Objective: To find out the antibacterial efficacy of Cuscuta reflexa (C. reflexa) and examine
the chemical composition of the essential oil from C. reflexa by gas chromatography and mass
spectrometry (GC-MS).
Methods: Antibacterial potency of C. reflexa plant extracts has been tested against Bacillus
subtilis IFO 3026, Sarcina lutea IFO 3232, Xanthomonas campestris IAM 1671, Escherichia coli
IFO 3007, Klebsiella pneumoniae ATTC 10031, Proteus vulgaris MTCC 321 and Pseudomonas
denitrificans KACC 32026 by disc diffusion assay. The chemical compositions of the essential
oil were analyzed by GC-MS.
Results: GC-MS analysis identified sixteen compounds from essential oil of C. reflexa, of
which piperitone (15.19%), caryophyllene (12.55%), β-elemene (10.35%), elemol (6.20%),
α-selinene (4.49%), α-humulene (3.98%) and thujone (5.18%) were the major compounds. ααBergamotene and juniper camphor were identified as the minor compounds. Dichloromethane
and petroleum ether extracts of C. reflexa showed a great potential of antibacterial activity
against all tested bacteria. Minimum inhibitory concentrationvalues of various extracts ranged
from 16 to 512 µg/mL.
Conclusions: These extracts showed good antibacterial activity and could lead to new
antibacterial drug designing.